SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. . application or budget. Microplate Readers. In vivo anti-malarial assay in mice. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeOpening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Donor filter: 620 nm. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. VersaMax reads 96-well and strip-well plates. MCE has not independently confirmed the accuracy of these methods. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. using SpectraMax i3 (Molecular devices, San Jose, USA). Samples were analyzed in triplicates. (1988) and recorded at 412 nm. Microplate Reader Shelf. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. 00. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Awareness Technology, Inc. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. \r plates can be used in the SPECTRAmax PLUS 384. 340 nm / 70 nm. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Italic and boldface type are used for emphasis. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Integration delay. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Introduction. edu Contact Phone: 216-368-8962. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Assay buffer was 50 m M Hepes, pH 7. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. TDP-43 stress granular cells were treated with 70% ice-cold. Briefly, cells were seeded into a. ). 5 mL of 1 M acetate buffer (pH 4. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. 2. 2 - 3. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). The plates were incubated for 4 additional hours at 37°C. 3 mm. Water – distilled water or equivalent. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax Microplate Readers. Vessel Sprout Formation Assay. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. These models can no longer be repaired. Driessen*,MaaikeA. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. , Sunnyvale, CA). ir Neuroprotection of a sesamin derivative, 1, 2-bis [(3-methoxy- phenyl) methyl] ethane-1, 2-dicaroxylic acid (MMEDA) againstEach SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. #N207) LAL Reagent Water (Lonza cat. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Although all samples initially were analyzed at the same volume, the volume of sample. reader (Molecular Devi ces). Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. 5), L-ascorbic acid (25. 30. 10× trypsin 0. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Values are expressed as the mean s ± SD of 3 separate experiments, each performed in triplicate. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. The plate was covered and incubated for one hour at room temperature. DNA extraction from blood meal ELISA homogenate. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Money Saved: $340. Bioz Stars score: 86/100, based on 1 PubMed citations. Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. Each experiment was performed in duplicate and repeated at. Immunofluorescence and Aggregation Analysis. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Anti-proteolytic activity. 20. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. 5 ng/ml; estradiol: 10 pg/ml. Stat Fax 3200 Microplate Reader. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. Number of flashes. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). #N207) LAL Reagent Water (Lonza cat. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. One plate carrier adapter is provided with the instrument. used in the SPECTRAmax PLUS. Statistical analysis. ) to the upper chamber, followed by measurement with a spectrophotometer (SpectraMax 340, Molecular Devices LLC, Sunnyvale, CA, USA) at 610 nm of media in the lower chamber after one hour . The absorbance was measured at 340 nm for 2 min at a 15 s time interval. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. The values were corrected for nonspecific background by subtracting the OD 650. View the Molecular Devices website. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The light features a slim profile to prevent. reader (Molecular Devi ces). Molecular Devices SpectraMax M5e Microplate Reader. ISM Equipment Classification (Group 1, Class A) This equipment is designated as. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. CD154 is an established indicator of antigen-reactive T helper cells in other species, but has not been characterized in horses. Wavelength range: 340-850 nm. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. ): 8. Cells were scraped from the plate and single cell suspensions were made by passing cells through 28G1/2 insulin syringes. To. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Upgradeable modules are also available including western blot, cell imaging, and fast. Dissolve 13. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. FlexStation® 3 Multi-Mode Microplate Reader. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. , Sunnyvale, CA). Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. ) at 490 nm. MOLECULAR DEVICE M5. Acceptor: 665/10 nm. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SoftMax Pro. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). Inter-assay variation was prevented by testing all of the samples on the same day. 003 OD Stray light ≤ 0. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Plates were washed with 1× KP solution and blocked with. , Sunnyvale, Calif. 85%. Details. The assays contained 0. PerkinElmer. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. 305 reference level saturation. 190–850 nm <± 1. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. SpectraMax Multi-Mode Microplate Readers. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. SpectraMax Injector Cartridge with SmartInject™ Technology. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. 6 (h) x 22. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. Lactate Dehydrogenase (LDH) Release. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). Absorbance was read at 450 nm using a Molecular Device Spectramax 340. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. Both readers feature an 8-channel read head for fast reads and. plates can be used in the SPECTRAmax PLUS 384. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Please refer to photos for further. Rate of fibrinolysis. D. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. Other technologies such as imaging. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. The cell survival. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. SpectraMax 190 reads 96-well and strip-well plates. Table 1. 313 reference gain check fail. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. 5 mL of 1 M acetate buffer (pH 4. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Isolation of RNA and RT-PCR. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. SpectraMAX 340. 5mM), and changes in OD 405 nm. 5 - 2. Details. Western blot. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. 7. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 4) was followed according to Vanacker et al. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Continuous kinetic monitoring of enzyme activity was performed on SPECTRAmax 340 or Flexstation 2-384 microplate reader (Molecular Devices, Sunnyvale, CA, USA) and controlled by the Softmax software. using SpectraMax i3 (Molecular devices, San Jose, USA). When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Quick View. 314 low lamp level warning. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. SPECTRAmax® 190. Emission. Following incubation for 30 min at room temperature, the reaction was quenched by addition of 100 μl stop solution (2 N H 2 SO 4) and absorbance was read using microplate reader (SpectraMax 340; Molecular Devices Co. Exhibit with us {{>currencyLabel}} Back. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). 260 280 300 320 340 360 Instrument: Molecular Devices SpectraMax 250 Instrument: LAMBDA 19/UV/VIS/NIR O. Or scan up to 384 wells at any time to determine the best assay conditions. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. Call 1-800-745-2710. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. StakMax. 7 macrophages were obtained from ATCC (Manassas, VA). the SpectraMax iD3 is a fantastic plate reader for a. 04. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). It includes both a lamp and a new lamp holder. Data were expressed as the mean LPS-treated cells (100%) vs. SpectraMax Multi-Mode Microplate Readers. Molecular Devices. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). 0 3. The area under the curve (AUC) for the comparison of the growth rates was determined using. , Sunnyvale, CA). Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. Donor filter: 616/10 nm. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. The metals were preincubated with A for 5 min when added before heme. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). , Sunnyvale, CA, USA) at a wavelength of 450 nm. 5. Microplate Readers. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. Product Keyword. 304 signal level saturation. Lactate Dehydrogenase (LDH) Release. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Molecular Devices Spectramax 340PC instructions. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. Cytotoxicity was determined by measuring the release of LDH. The SpectraMax® QuickDrop™ Micro-Volume Spectrophotometer . When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 5. 1% N-1-naphtylethylenediamine dihydrochloride, 2. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). Gerritsma,B. 190-1000 nm. Male 8 pin mini Din to Female 9D. Softmax PRO v5. Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 024). SpectraMAX 340 microtiter plate reader. measurements were performed on a SPECTRAmax-340 plate reader (Molecular Devices Inc. Six wells were assayed for each set of conditions and stadard deviations. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. 5 g/L-EDTA 0. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. We also offer depot Repair Service from our base of operations in Southern California (San Diego). The absorbance was measured at 340 nm for 2 min at a 15 s time interval. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). Crowder, Ziqian Zeng, Alissa N. 1 mL of culture media with 10% FBS. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. 0, MSD) or Spectramax 340 (Molecular Devices). The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Buffer (100 mmol L –1acetate buffer, pH 5. Call 1-800-745-2710. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. 0200-7027. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. The contents of the. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. Centrifuge – Eppendorf 5417C with rotor FA 45–30–11. All strains were tested three times and the results averaged. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 97), and each plate contained at least six positive and six negative controls. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). Quick View. One plate carrier adapter is provided with the instrument. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the visible range. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. 0200-0729. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Products : SpectraMax Paradigm reader, SpectraMax Plus 384 reader, SpectraMax 250 (Obsolete), SpectraMax L reader, SpectraMax 340PC 384 (Obsolete), Gemini EM reader, Gemini XPS reader, SpectraMax 340 (Obsolete). 306 plate air cal. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. 316 grating motor driver faulty. Service parts for these models are not being produced and are no longer in stock. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. The growth. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. 340 nm. 2. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Awareness Technology Inc. Each experiment was performed in duplicate and repeated at. The reaction was stopped with 1 M hydrochloric acid after 15 min. As a common indicator of equine T cell activation, cytokine induction. The estimation of monodehydroascorbate reductase (MDHAR) (EC 1. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Streamline GLP/GMP compliance. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. ScanLater Western Blot Detection Cartridge. N-34-LB. The ABS reader ranges from. edu Contact Phone: 216-368-8962. using SpectraMax i3 (Molecular devices, San Jose, USA). Details. 003 OD Stray light ≤ 0. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. Example: [Drawer]. High fg levels of Streptavidin High fg levels of Streptavidin Exceeds Cisbio certification requirements TRF-based with 340/80 nm EX and 616/10 nm EM AlphaScreen AlphaScreen and AlphaLisa detection using 1 W 680 nm. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Versteegh, Yoran H. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. SPECTRAmax® 190. ). Microplate Spectrophotometer Operator’s Manual. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 190–850 nm <± 1.